NITROGEN FIXATION CHARACTERISTICS OF AZOLLA CAROLINIANA UNDER THE INFLUENCE OF HEAVY METAL (CR⁶⁺)
DOI:
https://doi.org/10.5281/zenodo.18413154Ключевые слова:
Azolla caroliniana, nitrogen fixation, chromium (Cr⁶⁺), nutrient medium, biomass, acetylene reduction method, Anabaena azollae.Аннотация
This study investigated the nitrogen fixation activity of the aquatic plant Azolla caroliniana in different nutrient
media (D and W) under the influence of various concentrations of hexavalent chromium (Cr⁶⁺). During the experiments,
biomass production, growth dynamics, and nitrogen fixation activity of Azolla were evaluated using the acetylene
reduction method. The results showed that in chromium-free nutrient media, nitrogen fixation activity gradually increased
over 10–30 days and exhibited a declining trend after day 35. Nitrogen fixation activity in the W-type nutrient medium was
higher compared to the D medium. Although increasing chromium concentrations significantly reduced nitrogen fixation
activity, the process did not completely cease even at high concentrations (3 mg/L). The results indicate that Azolla
caroliniana is a relatively stable biological object in water environments contaminated with heavy metals.
Библиографические ссылки
Aravind P., Prasad MNV., Malec P., Waloszek A., Strzałka K. 2009. Zinc protects Ceratophyllum demersum L. (freefloating
hydrophyte) against reactive oxygen species induced by cadmium. J Trace Elem Med Biol 23:50–60.
Arimoro, F., Chukwujindu, I. &Osiobe, O. 2008 Effects ofindustrial waste water on the physical and chemicalcharacteristics
of a tropical coastal river. Research Journal ofEnvironmental Sciences 2 (3), 209–220.
Arora, A., A. Sood, and P.K. Singh. 2004. Hyperaccumulation of cadmium and nickel by Azolla species. Indian Journal
of Plant Physiology 3: 302–304.
Arora A., Saxena S. 2005. Cultivation of Azolla microphylla biomass on secondary-treated Delhi municipal effluents.
Biomass Bioenergy 29:60–64.
Arora A., Saxena T., Sharma D.K. 2006. Tolerance and phytoaccumulation of chromium by three Azolla species. World
Journal of Microbiology & Biotechnology 22: 97–100.
Ashraf H., Matto FA., Ganai AM., Reshi IM., Sheikh FA. 2015. Effect of replacement of mustard oil cake with Azolla
(Azolla pinnata) meal on growth performance of broilers and economics of feeding under temperate conditions. Indian
J Anim Nutr 32:325–328.
Basak B., Pramanik MAH., Rahman MS., Tarafdar SU., Roy BC. 2002. Azolla (Azolla pinnata) as a feed ingredient in
broiler ration. Int J Poult Sci 1:29–34.
Basset, N., Katsou, E., Frison, N., Malamis, S., Dosta, J. &Fatone, F. 2016 Integrating the selection of PHA storing
biomass andnitrogen removal via nitrite in the main wastewater treatmentline. Bioresource Technology 200, 820–829.
Babu D.J., Sumalatha B., Venkateswarulu T.C., Das K.M., Kodali V.P. 2014. Kinetic, equilibrium and thermodynamic
studies of biosorption of Chromium (VI) from aqueous solutions using Azolla Filiculoidus. Journal of Pure and Applied
Microbiology, 8(4), 3107–3116.
Benaroya, B.O., V. Tzin, E. Tel-Or, and E. Zamski. 2004. Lead accumulation in the aquatic fern Azolla filiculoides.
Plant Physiology and Biochemistry 42: 639–645.
Bennicelli R., Stezpniewska C.B., Banach A., Szajnocha K., Ostrowski J. 2004. The ability of Azolla caroliniana to
remove heavy metals (Hg(II), Cr(III), Cr(VI)) from municipal waste water. Chemosphere 55: 141–146.
Bottomley W. B. 1920. The effect of organic matter on the growth of various water plants in culture solution. Ann. Bot.
: 353-365.
Brouwer P., van der Werf A., Schluepmann H., Reichart G-J., Nierop KG. 2015. Lipid yield and composition of Azolla
filiculoides and the implications for biodiesel production. Bioenergy Res. Рр. 1–9.
Cohen RR. 2006. Use of microbes for cost reduction of metal removal from metals and mining industry waste streams.
J Clean Prod 14: 1146–1157.
Hamdi YA 1982. Applicationofnitrogen-fixingsystemsinsoilimprovement and management. Food Agric Org. Pp. 25-30.
Hardy D.W., Halstein R., Jakson E., Buens R.S. 1968. C2H2 - C2H4 asay to N2 fixation laboratory and field evaluation.
Plant Phys. V.43. Pp. 9-13.
Hardy R.W.F., Burns R.C., Herbert R.R., Holsten R.D., Jackson E.K. 1971. Biological nitrogen fixation: a key to world
protein. // Plant and Soil/ Special vol. - Pp. 561-590.
Kangmin L. 1988. Rice-fish culture in China: a review. Aquaculture 71: 173–186.
Mosa, K. A., Saadoun, I., Kumar, K., Helmy, M. &Dhankher,O. P. 2016 Potential biotechnological strategies for
thecleanup of heavy metals and metalloids. Frontiers in PlantScience 7, 303–303.
Morgan-Sagastume, F., Hjort, M., Cirne, D., Gérardin, F., Lacroix,S., Gaval, G., Karabegovic, L., Alexandersson,
T., Johansson,P., Karlsson, A., Bengtsson, S., Arcos-Hernandez, M.,Magnusson, P. &Werker, A. 2015 Integrated
production ofpolyhydroxyalkanoates (PHAs) with municipal wastewaterand sludge treatment at pilot scale. Bioresource
Technology181, 78–89
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