NITROGEN FIXATION CHARACTERISTICS OF AZOLLA CAROLINIANA UNDER THE INFLUENCE OF HEAVY METAL (CR⁶⁺)
DOI:
https://doi.org/10.5281/zenodo.18413154Keywords:
Azolla caroliniana, nitrogen fixation, chromium (Cr⁶⁺), nutrient medium, biomass, acetylene reduction method, Anabaena azollae.Abstract
This study investigated the nitrogen fixation activity of the aquatic plant Azolla caroliniana in different nutrient
media (D and W) under the influence of various concentrations of hexavalent chromium (Cr⁶⁺). During the experiments,
biomass production, growth dynamics, and nitrogen fixation activity of Azolla were evaluated using the acetylene
reduction method. The results showed that in chromium-free nutrient media, nitrogen fixation activity gradually increased
over 10–30 days and exhibited a declining trend after day 35. Nitrogen fixation activity in the W-type nutrient medium was
higher compared to the D medium. Although increasing chromium concentrations significantly reduced nitrogen fixation
activity, the process did not completely cease even at high concentrations (3 mg/L). The results indicate that Azolla
caroliniana is a relatively stable biological object in water environments contaminated with heavy metals.
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